Dot blot technique principle , procedure and application
Dot blot technique principle , procedure and application
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One such immunodetection method is widely known as Dot Blot The method involves applying small volumes of antigens or test samples on protein binding membrane;
The units incorporate precision lapped surfaces to ensure uniform blotting membrane contact and a leak-proof gasket These prevent lateral transfer of samples – A dot blot is a technique in molecular biology used to detect proteins It represents a simplification of the western blot method, with the
aluminium t slot Dot Blot · Membrane Preparation · Block · Wash · Primary Antibody Incubation · Wash · Secondary Antibody Incubation · Wash · Detection Our simple slot blot protocol highlights pre-treatment of HA samples with moderate concentrations of denaturant to maximally expose the fusion
